4RNP

BACTERIOPHAGE T7 RNA POLYMERASE, HIGH SALT CRYSTAL FORM, LOW TEMPERATURE DATA, ALPHA-CARBONS ONLY

Replaces:  3RNPReplaces:  2RNP

Summary for 4RNP

• Entry DOI: 10.2210/pdb4rnp/pdb
• Descriptor: RNA POLYMERASE (1 entity in total)
• Functional Keywords: nucleotidyltransferase, rna polymerase, transcription
• Biological source: Enterobacteria phage T7
• Total number of polymer chains: 3
• Total formula weight: 296952.68

Authors

Liu, Z.J.,Sousa, R.,Rose, J.P.,Wang, B.C. (deposition date: 1997-09-11, release date: 1997-12-03, Last modification date: 2024-02-28)

Primary citation

Sousa, R.,Chung, Y.J.,Rose, J.P.,Wang, B.C.
Crystal structure of bacteriophage T7 RNA polymerase at 3.3 A resolution.
Nature, 364:593-599, 1993

PubMed Abstract: The crystal structure of T7 RNA polymerase reveals a molecule organized around a cleft that can accommodate a double-stranded DNA template. A portion (approximately 45%) of the molecule displays extensive structural homology to the polymerase domain of Klenow fragment and more limited homology to the human immunodeficiency virus HIV-1 reverse transcriptase. A comparison of the structures and sequences of these polymerases identifies structural elements that may be responsible for discriminating between ribonucleotide and deoxyribonucleotide substrates, and RNA and DNA templates. The relative locations of the catalytic site and a specific promoter recognition residue allow the orientation of the polymerase on the template to be defined.

PubMed: 7688864
DOI: 10.1038/364593a0

Experimental method

X-RAY DIFFRACTION (3 Å)