4RH0

Spore photoproduct lyase C140A/S76C mutant with bound AdoMet

Summary for 4RH0

• Entry DOI: 10.2210/pdb4rh0/pdb
• Related: 4FHC 4FHD 4FHE 4FHF 4FHG 4K9R 4RH1
• Descriptor: Spore photoproduct lyase, [(3S)-3-amino-4-hydroxy-4-oxo-butyl]-[[(2S,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxy-oxolan-2-yl]methyl]-methyl-selanium, SULFATE ION, ... (6 entities in total)
• Functional Keywords: radical adomet enzyme, radical sam enzyme, dna repair, tim barrel, dna lyase, lyase
• Biological source: Geobacillus thermodenitrificans NG80-2
• Total number of polymer chains: 1
• Total formula weight: 44396.34

Authors

Benjdia, A.,Heil, K.,Winkler, A.,Carell, T.,Schlichting, I. (deposition date: 2014-10-01, release date: 2014-10-22, Last modification date: 2024-02-28)

Primary citation

Benjdia, A.,Heil, K.,Winkler, A.,Carell, T.,Schlichting, I.
Rescuing DNA repair activity by rewiring the H-atom transfer pathway in the radical SAM enzyme, spore photoproduct lyase.
Chem.Commun.(Camb.), 50:14201-14204, 2014

PubMed Abstract: The radical SAM enzyme, spore photoproduct lyase, requires an H-atom transfer (HAT) pathway to catalyze DNA repair. By rational engineering, we demonstrate that it is possible to rewire its HAT pathway, a first step toward the development of novel catalysts based on the radical SAM enzyme scaffold.

PubMed: 25285338
DOI: 10.1039/c4cc05158k

Experimental method

X-RAY DIFFRACTION (2.1 Å)