4RGF

Crystal structure of the in-line aligned env22 twister ribozyme soaked with Mn2+

Summary for 4RGF

• Entry DOI: 10.2210/pdb4rgf/pdb
• Related: 4RGE
• Descriptor: env22 twister ribozyme, MANGANESE (II) ION, POTASSIUM ION, ... (5 entities in total)
• Functional Keywords: pseudoknot, stems, cleavage, rna
• Biological source: Synthetic
• Total number of polymer chains: 3
• Total formula weight: 55208.26

Authors

Ren, A.,Rajashankar, K.R.,Simanshu, D.,Patel, D. (deposition date: 2014-09-30, release date: 2014-12-03, Last modification date: 2024-02-28)

Primary citation

Ren, A.,Kosutic, M.,Rajashankar, K.R.,Frener, M.,Santner, T.,Westhof, E.,Micura, R.,Patel, D.J.
In-line alignment and Mg(2+) coordination at the cleavage site of the env22 twister ribozyme.
Nat Commun, 5:5534-5534, 2014

PubMed Abstract: Small self-cleaving nucleolytic ribozymes contain catalytic domains that accelerate site-specific cleavage/ligation of phosphodiester backbones. We report on the 2.9-Å crystal structure of the env22 twister ribozyme, which adopts a compact tertiary fold stabilized by co-helical stacking, double-pseudoknot formation and long-range pairing interactions. The U-A cleavage site adopts a splayed-apart conformation with the modelled 2'-O of U positioned for in-line attack on the adjacent to-be-cleaved P-O5' bond. Both an invariant guanosine and a Mg(2+) are directly coordinated to the non-bridging phosphate oxygens at the U-A cleavage step, with the former positioned to contribute to catalysis and the latter to structural integrity. The impact of key mutations on cleavage activity identified an invariant guanosine that contributes to catalysis. Our structure of the in-line aligned env22 twister ribozyme is compared with two recently reported twister ribozymes structures, which adopt similar global folds, but differ in conformational features around the cleavage site.

PubMed: 25410397
DOI: 10.1038/ncomms6534

Experimental method

X-RAY DIFFRACTION (3.2008 Å)