4REQ

Methylmalonyl-COA Mutase substrate complex

4REQ の概要

• エントリーDOI: 10.2210/pdb4req/pdb
• 分子名称: METHYLMALONYL-COA MUTASE, COBALAMIN, SUCCINYL-COENZYME A, ... (8 entities in total)
• 機能のキーワード: isomerase, mutase, intramolecular transferase
• 由来する生物種: Propionibacterium freudenreichii subsp. shermanii; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 306138.08

構造登録者

Evans, P.R.,Mancia, F. (登録日: 1998-06-17, 公開日: 1999-01-13, 最終更新日: 2024-05-22)

主引用文献

Mancia, F.,Evans, P.R.
Conformational changes on substrate binding to methylmalonyl CoA mutase and new insights into the free radical mechanism.
Structure, 6:711-720, 1998

PubMed Abstract: Methylmalonyl CoA mutase catalyses the interconversion of succinyl CoA and methylmalonyl CoA via a free radical mechanism. The enzyme belongs to a family of enzymes that catalyse intramolecular rearrangement reactions in which a group and a hydrogen atom on adjacent carbons are exchanged. These enzymes use the cofactor adenosylcobalamin (coenzyme B12) which breaks to form an adenosyl radical, thus initiating the reaction. Determination of the structure of substrate-free methylmalonyl CoA mutase was initiated to provide further insight into the mechanism of radical formation.

PubMed: 9655823
DOI: 10.1016/S0969-2126(98)00073-2

実験手法

X-RAY DIFFRACTION (2.2 Å)