4R8U

S-SAD structure of DINB-DNA Complex

Summary for 4R8U

• Entry DOI: 10.2210/pdb4r8u/pdb
• Descriptor: DNA polymerase IV, DNA, MAGNESIUM ION, ... (8 entities in total)
• Functional Keywords: nucleotidyl transferase, dna polymerase, transferase-dna complex, transferase/dna
• Biological source: Escherichia coli K-12; More
• Cellular location: Cytoplasm : Q47155 Q47155
• Total number of polymer chains: 6
• Total formula weight: 98111.83

Authors

Kottur, J.,Nair, D.T.,Weinert, T.,Oligeric, V.,Wang, M. (deposition date: 2014-09-03, release date: 2015-01-14, Last modification date: 2024-03-20)

Primary citation

Weinert, T.,Olieric, V.,Waltersperger, S.,Panepucci, E.,Chen, L.,Zhang, H.,Zhou, D.,Rose, J.,Ebihara, A.,Kuramitsu, S.,Li, D.,Howe, N.,Schnapp, G.,Pautsch, A.,Bargsten, K.,Prota, A.E.,Surana, P.,Kottur, J.,Nair, D.T.,Basilico, F.,Cecatiello, V.,Pasqualato, S.,Boland, A.,Weichenrieder, O.,Wang, B.C.,Steinmetz, M.O.,Caffrey, M.,Wang, M.
Fast native-SAD phasing for routine macromolecular structure determination
Nat.Methods, 12:131-133, 2015

PubMed Abstract: We describe a data collection method that uses a single crystal to solve X-ray structures by native SAD (single-wavelength anomalous diffraction). We solved the structures of 11 real-life examples, including a human membrane protein, a protein-DNA complex and a 266-kDa multiprotein-ligand complex, using this method. The data collection strategy is suitable for routine structure determination and can be implemented at most macromolecular crystallography synchrotron beamlines.

PubMed: 25506719
DOI: 10.1038/nmeth.3211

Experimental method

X-RAY DIFFRACTION (2.3 Å)