4R7N

Fab C2E3

Summary for 4R7N

• Entry DOI: 10.2210/pdb4r7n/pdb
• Related: 4R7D
• Descriptor: Fab C2E3 Heavy chain, Fab C2E3 Light chain (2 entities in total)
• Functional Keywords: tlr4 antibody, immune system
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 20
• Total formula weight: 478243.75

Authors

Loyau, J.,Didelot, G.,Malinge, P.,Ravn, U.,Magistrelli, G.,Depoisier, J.F.,Kosco-Vilbois, M.,Fischer, N.,Thore, S.,Rousseau, F. (deposition date: 2014-08-28, release date: 2015-07-29, Last modification date: 2015-08-26)

Primary citation

Loyau, J.,Didelot, G.,Malinge, P.,Ravn, U.,Magistrelli, G.,Depoisier, J.F.,Pontini, G.,Poitevin, Y.,Kosco-Vilbois, M.,Fischer, N.,Thore, S.,Rousseau, F.
Robust Antibody-Antigen Complexes Prediction Generated by Combining Sequence Analyses, Mutagenesis, In Vitro Evolution, X-ray Crystallography and In Silico Docking.
J.Mol.Biol., 427:2647-2662, 2015

PubMed Abstract: Hu 15C1 is a potent anti-human Toll-like receptor 4 (TLR4) neutralizing antibody. To better understand the molecular basis of its biological activity, we used a multidisciplinary approach to generate an accurate model of the Hu 15C1-TLR4 complex. By combining site-directed mutagenesis, in vitro antibody evolution, affinity measurements and X-ray crystallography of Fab fragments, we identified key interactions across the Hu 15C1-TLR4 interface. These contact points were used as restraints to predict the structure of the Fab region of Hu 15C1 bound to TLR4 using computational molecular docking. This model was further evaluated and validated by additional site-directed mutagenesis studies. The predicted structure of the Hu 15C1-TLR4 complex indicates that the antibody antagonizes the receptor dimerization necessary for its activation. This study exemplifies how iterative cycles of antibody engineering can facilitate the discovery of components of antibody-target interactions.

PubMed: 26013163
DOI: 10.1016/j.jmb.2015.05.016

Experimental method

X-RAY DIFFRACTION (3.45 Å)