Ligand-induced aziridine-formation at subunit beta5 of the yeast 20S proteasome

Summary for 4R17

Related1RYP 4R18
DescriptorProteasome subunit alpha type-2, Proteasome subunit alpha type-3, Proteasome subunit alpha type-4, ... (17 entities in total)
Functional Keywordsproteasome, drug development, binding analysis, umpolung, crosslink, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
Biological sourceSaccharomyces cerevisiae S288c (Baker's yeast)
Cellular locationCytoplasm P23639 P23638 P40303 P32379 P40302 P21242 P21243 P25043 P25451 P22141 P30656 P23724 P30657 P38624
Total number of polymer chains28
Total molecular weight731520.33
Dubiella, C.,Cui, H.,Gersch, M.,Brouwer, A.J.,Sieber, S.A.,Krueger, A.,Liskamp, R.,Groll, M. (deposition date: 2014-08-04, release date: 2014-10-15, Last modification date: 2014-11-05)
Primary citation
Dubiella, C.,Cui, H.,Gersch, M.,Brouwer, A.J.,Sieber, S.A.,Kruger, A.,Liskamp, R.M.,Groll, M.
Selective inhibition of the immunoproteasome by ligand-induced crosslinking of the active site.
Angew.Chem.Int.Ed.Engl., 53:11969-11973, 2014
PubMed: 25244435 (PDB entries with the same primary citation)
DOI: 10.1002/anie.201406964
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.20340.3%6.9%3.6%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 4r17
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Molmil generated image of 4r17
rotated about x axis by 90°
Molmil generated image of 4r17
rotated about y axis by 90°