4R0I

CRYSTAL STRUCTURE of MATRIPTASE in COMPLEX WITH INHIBITOR

Summary for 4R0I

• Entry DOI: 10.2210/pdb4r0i/pdb
• Descriptor: Suppressor of tumorigenicity 14 protein, SERINE PROTEASE, MATRIPTASE, MEMBRANE-TYPE SERINE PROTEASE 1, MT-SP1, 3-({(2S)-3-[4-(2-aminoethyl)piperidin-1-yl]-2-[(naphthalen-2-ylsulfonyl)amino]-3-oxopropyl}oxy)benzenecarboximidamide, ... (4 entities in total)
• Functional Keywords: hydrolase, matriptase, complex structure, trypsin-like serine proteinase fold, protease, small molecule inhibitor
• Biological source: Homo sapiens (human); More
• Cellular location: Membrane ; Single-pass type II membrane protein : Q9Y5Y6 Q9Y5Y6
• Total number of polymer chains: 2
• Total formula weight: 27435.96

Authors

Rao, K.N.,Ashok, K.N.,Chakshusmathi, G.,Rajeev, G.,Subramanya, H. (deposition date: 2014-07-31, release date: 2015-02-11, Last modification date: 2024-11-13)

Primary citation

Goswami, R.,Wohlfahrt, G.,Mukherjee, S.,Ghadiyaram, C.,Nagaraj, J.,Satyam, L.K.,Subbarao, K.,Gopinath, S.,Krishnamurthy, N.R.,Subramanya, H.S.,Ramachandra, M.
Discovery of O-(3-carbamimidoylphenyl)-l-serine amides as matriptase inhibitors using a fragment-linking approach
Bioorg.Med.Chem.Lett., 25:616-620, 2015

PubMed Abstract: Matriptase is a cell-surface trypsin-like serine protease of epithelial origin, which cleaves and activates proteins including hepatocyte growth factor/scatter factor and proteases such as uPA, which are involved in the progression of various cancers. Here we report a fragment-linking approach, which led to the discovery of O-(3-carbamimidoylphenyl)-l-serine amides as potent matriptase inhibitors. The co-crystal structure of one of the potent inhibitors, 6 in complex with matriptase catalytic domain validated the working hypothesis guiding the development of this congeneric series and revealed the structural basis for matriptase inhibition. Replacement of a naphthyl group in 6 with 2,4,6-tri-isopropyl phenyl resulted in 10 with improved matriptase inhibition, which exhibited significant primary tumor growth inhibition in a mouse model of prostate cancer. Compounds such as 10, identified using a fragment-linking approach, can be explored further to understand the role of matriptase as a drug target in cancer and inflammation.

PubMed: 25556099
DOI: 10.1016/j.bmcl.2014.12.008

Experimental method

X-RAY DIFFRACTION (1.9 Å)