4QVA

Unliganded crystal structure of Feline Norovirus P Domain co-crystallized with N-glycolylneuraminic acid

Summary for 4QVA

• Entry DOI: 10.2210/pdb4qva/pdb
• Related: 4QUZ 4QV2
• Descriptor: VP1 (2 entities in total)
• Functional Keywords: feline norovirus, viral capsid protein, p domain, protruding domain, viral protein
• Biological source: Norovirus cat/GIV.2/CU081210E/USA/2010
• Total number of polymer chains: 2
• Total formula weight: 73325.97

Authors

Singh, B.K.,Hansman, G.S. (deposition date: 2014-07-14, release date: 2014-11-19, Last modification date: 2024-03-20)

Primary citation

Singh, B.K.,Glatt, S.,Ferrer, J.L.,Koromyslova, A.D.,Leuthold, M.M.,Dunder, J.,Hansman, G.S.
Structural analysis of a feline norovirus protruding domain.
Virology, 474:181-185, 2015

PubMed Abstract: Norovirus infects different animals, including humans, mice, dogs, and cats. Here, we show an X-ray crystal structure of a feline GIV.2 norovirus capsid-protruding (P) domain to 2.35Å resolution. The feline GIV.2 P domain was reminiscent of human norovirus P domains, except for a novel P2 subdomain α-helix and an extended P1 subdomain interface loop. These new structural features likely obstructed histo-blood group antigens, which are attachment factors for human norovirus, from binding at the equivalent sites on the feline GIV.2 P domain. Additionally, an ELISA showed that the feline GIV.2 was antigenically distinct from a human GII.10 norovirus.

PubMed: 25463616
DOI: 10.1016/j.virol.2014.10.028

Experimental method

X-RAY DIFFRACTION (2.18 Å)