4QN7

Crystal structure of neuramnidase N7 complexed with Oseltamivir

4QN7 の概要

• エントリーDOI: 10.2210/pdb4qn7/pdb
• 関連するPDBエントリー: 4QN3 4QN4 4QN5 4QN6
• 分子名称: Neuraminidase, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• 機能のキーワード: 6-bladed beta-propeller, hydrolase, calcium binding, glycosylation
• 由来する生物種: Influenza A virus (A/mallard/ALB/196/1996(H10N7))
• 細胞内の位置: Virion membrane: Q20R18
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 89023.30

構造登録者

Sun, X.,Li, Q.,Wu, Y.,Liu, Y.,Qi, J.,Vavricka, C.J.,Gao, G.F. (登録日: 2014-06-17, 公開日: 2014-07-30, 最終更新日: 2023-11-08)

主引用文献

Sun, X.,Li, Q.,Wu, Y.,Wang, M.,Liu, Y.,Qi, J.,Vavricka, C.J.,Gao, G.F.
Structure of influenza virus N7: the last piece of the neuraminidase "jigsaw" puzzle.
J.Virol., 88:9197-9207, 2014

PubMed Abstract: There are nine subtypes of influenza A virus neuraminidase (NA), N1 to N9. In addition, influenza B virus also contains NA, and there are two influenza virus NA-like molecules, N10 and N11, which were recently identified from bats. Crystal structures for all of these proteins have been solved, with the exception of N7, and there is no published report of N6, although a structure has been deposited in the Protein Data Bank. Here, we present the N7 and N6 structures at 2.1 Å and 1.8 Å, respectively. Structural comparison of all NA subtypes shows that both N7 and N6 highly resemble typical group 2 NA structures with some special characteristics, including an additional cavity adjacent to their active sites formed by novel 340-loop conformations. Comparative analysis also revealed new structural insights into the N-glycosylation, calcium binding, and second sialic acid binding site of influenza virus NA. This comprehensive study is critical for understanding the complexity of the most successful influenza drug target and for the structure-based design of novel influenza inhibitors.

PubMed: 24899180
DOI: 10.1128/JVI.00805-14

実験手法

X-RAY DIFFRACTION (2.302 Å)