4QIW

Crystal structure of euryarchaeal RNA polymerase from Thermococcus kodakarensis

Summary for 4QIW

• Entry DOI: 10.2210/pdb4qiw/pdb
• Descriptor: DNA-directed RNA polymerase, DNA-directed RNA polymerase subunit N, DNA-directed RNA polymerase subunit P, ... (14 entities in total)
• Functional Keywords: transcription, dna-directed rna polymerase
• Biological source: Thermococcus kodakarensis; More
• Total number of polymer chains: 22
• Total formula weight: 762518.19

Authors

Jun, S.-H.,Murakami, K.S. (deposition date: 2014-06-02, release date: 2014-10-08, Last modification date: 2024-10-30)

Primary citation

Jun, S.H.,Hirata, A.,Kanai, T.,Santangelo, T.J.,Imanaka, T.,Murakami, K.S.
The X-ray crystal structure of the euryarchaeal RNA polymerase in an open-clamp configuration.
Nat Commun, 5:5132-5132, 2014

PubMed Abstract: The archaeal transcription apparatus is closely related to the eukaryotic RNA polymerase II (Pol II) system. Archaeal RNA polymerase (RNAP) and Pol II evolved from a common ancestral structure and the euryarchaeal RNAP is the simplest member of the extant archaeal-eukaryotic RNAP family. Here we report the first crystal structure of euryarchaeal RNAP from Thermococcus kodakarensis (Tko). This structure reveals that the clamp domain is able to swing away from the main body of RNAP in the presence of the Rpo4/Rpo7 stalk by coordinated movements of these domains. More detailed structure-function analysis of yeast Pol II and Tko RNAP identifies structural additions to Pol II that correspond to the binding sites of Pol II-specific general transcription factors including TFIIF, TFIIH and Mediator. Such comparisons provide a framework for dissecting interactions between RNAP and these factors during formation of the pre-initiation complex.

PubMed: 25311937
DOI: 10.1038/ncomms6132

Experimental method

X-RAY DIFFRACTION (3.5 Å)