4PWS

Crystal structure of secreted proline rich antigen MTC28 (Rv0040c) at 2.15 A with bound chloride from Mycobacterium tuberculosis

Summary for 4PWS

• Entry DOI: 10.2210/pdb4pws/pdb
• Descriptor: Proline-rich 28 kDa antigen, CHLORIDE ION (3 entities in total)
• Functional Keywords: probable lipoprotein lpqn, immune system
• Biological source: Mycobacterium tuberculosis
• Total number of polymer chains: 1
• Total formula weight: 29935.18

Authors

Kundu, P.,Biswas, R.,Mukherjee, S.,Reinhard, L.,Mueller-dieckmann, J.,Weiss, M.S.,Das, A.K. (deposition date: 2014-03-21, release date: 2015-03-25, Last modification date: 2023-11-08)

Primary citation

Kundu, P.,Biswas, R.,Mukherjee, S.,Reinhard, L.,Dutta, A.,Mueller-Dieckmann, J.,Weiss, M.S.,Pal, N.K.,Das, A.K.
Structure-based Epitope Mapping of Mycobacterium tuberculosis Secretary Antigen MTC28
J.Biol.Chem., 291:13943-13954, 2016

PubMed Abstract: Secretary proteins of Mycobacterium tuberculosis are key players of the mycobacterial infection pathway. MTC28 is a 28-kDa proline-rich secretary antigen of Mycobacterium tuberculosis and is only conserved in pathogenic strains of mycobacteria. Here we report the crystal structure of MTC28 at 2.8- and 2.15-Å resolutions for the structure-based epitope design. MTC28 shares a "mog1p"-fold consisting of seven antiparallel β strands stacked between α helices. Five probable epitopes have been located on a solvent-accessible flexible region by computational analysis of the structure of MTC28. Simultaneously, the protein is digested with trypsin and the resulting fragments are purified by HPLC. Such 10 purified peptide fragments are screened against sera from patients infected with pulmonary tuberculosis (PTB). Two of these 10 fragments, namely (127)ALDITLPMPPR(137) and (138)WTQVPDPNVPDAFVVIADR(156),are found to be major immunogenic epitopes that are localized on the outer surface of the protein molecule and are part of a single continuous epitope that have been predicted in silico Mutagenesis and antibody inhibition studies are in accordance with the results obtained from epitope mapping.

PubMed: 27189947
DOI: 10.1074/jbc.M116.726422

Experimental method

X-RAY DIFFRACTION (2.15 Å)