4PQK

C-Terminal domain of DNA binding protein

Summary for 4PQK

• Entry DOI: 10.2210/pdb4pqk/pdb
• Related: 4PQL 4PT7 4PTA
• Related PRD ID: PRD_900009
• Descriptor: Maltose ABC transporter periplasmic protein, Truncated replication protein RepA, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose (2 entities in total)
• Functional Keywords: dna binding protein
• Biological source: Escherichia coli; More
• Total number of polymer chains: 4
• Total formula weight: 221867.86

Authors

Schumacher, M.A.,Chinnam, N.,Tonthat, N.K. (deposition date: 2014-03-03, release date: 2014-06-18, Last modification date: 2024-02-28)

Primary citation

Schumacher, M.A.,Tonthat, N.K.,Kwong, S.M.,Chinnam, N.B.,Liu, M.A.,Skurray, R.A.,Firth, N.
Mechanism of staphylococcal multiresistance plasmid replication origin assembly by the RepA protein.
Proc.Natl.Acad.Sci.USA, 111:9121-9126, 2014

PubMed Abstract: The staphylococcal multiresistance plasmids are key contributors to the alarming rise in bacterial multidrug resistance. A conserved replication initiator, RepA, encoded on these plasmids is essential for their propagation. RepA proteins consist of flexibly linked N-terminal (NTD) and C-terminal (CTD) domains. Despite their essential role in replication, the molecular basis for RepA function is unknown. Here we describe a complete structural and functional dissection of RepA proteins. Unexpectedly, both the RepA NTD and CTD show similarity to the corresponding domains of the bacterial primosome protein, DnaD. Although the RepA and DnaD NTD both contain winged helix-turn-helices, the DnaD NTD self-assembles into large scaffolds whereas the tetrameric RepA NTD binds DNA iterons using a newly described DNA binding mode. Strikingly, structural and atomic force microscopy data reveal that the NTD tetramer mediates DNA bridging, suggesting a molecular mechanism for origin handcuffing. Finally, data show that the RepA CTD interacts with the host DnaG primase, which binds the replicative helicase. Thus, these combined data reveal the molecular mechanism by which RepA mediates the specific replicon assembly of staphylococcal multiresistant plasmids.

PubMed: 24927575
DOI: 10.1073/pnas.1406065111

Experimental method

X-RAY DIFFRACTION (3.401 Å)