4PMI

Crystal structure of Rev and Rev-response-element RNA complex

4PMI の概要

• エントリーDOI: 10.2210/pdb4pmi/pdb
• 分子名称: Rev-Response-Element RNA, Protein Rev, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: protein-rna complex, helix-loop-helix, hiv, nuclear export, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Human immunodeficiency virus type 1 group M subtype B (HIV-1); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 29795.51

構造登録者

Jayaraman, B.,Crosby, D.C.,Homer, C.,Ribeiro, I.,Mavor, D.,Frankel, A.D. (登録日: 2014-05-21, 公開日: 2014-12-24, 最終更新日: 2023-09-27)

主引用文献

Jayaraman, B.,Crosby, D.C.,Homer, C.,Ribeiro, I.,Mavor, D.,Frankel, A.D.
RNA-directed remodeling of the HIV-1 protein Rev orchestrates assembly of the Rev-Rev response element complex.
Elife, 4:e04120-e04120, 2014

PubMed Abstract: The HIV-1 protein Rev controls a critical step in viral replication by mediating the nuclear export of unspliced and singly-spliced viral mRNAs. Multiple Rev subunits assemble on the Rev Response Element (RRE), a structured region present in these RNAs, and direct their export through the Crm1 pathway. Rev-RRE assembly occurs via several Rev oligomerization and RNA-binding steps, but how these steps are coordinated to form an export-competent complex is unclear. Here, we report the first crystal structure of a Rev dimer-RRE complex, revealing a dramatic rearrangement of the Rev-dimer upon RRE binding through re-packing of its hydrophobic protein-protein interface. Rev-RNA recognition relies on sequence-specific contacts at the well-characterized IIB site and local RNA architecture at the second site. The structure supports a model in which the RRE utilizes the inherent plasticity of Rev subunit interfaces to guide the formation of a functional complex.

PubMed: 25486594
DOI: 10.7554/eLife.04120

実験手法

X-RAY DIFFRACTION (3.2 Å)