4PKM
Crystal Structure of Bacillus thuringiensis Cry51Aa1 Protoxin at 1.65 Angstroms Resolution
Summary for 4PKM
| Entry DOI | 10.2210/pdb4pkm/pdb |
| Descriptor | Cry51Aa1, GLYCEROL, SULFATE ION, ... (5 entities in total) |
| Functional Keywords | bacterial toxins, cry toxins, pore-forming toxins, beta-pore-forming toxins, beetles, insecticidal toxins, pro-toxins, toxin |
| Biological source | Bacillus thuringiensis |
| Total number of polymer chains | 1 |
| Total formula weight | 34420.19 |
| Authors | |
| Primary citation | Xu, C.,Chinte, U.,Chen, L.,Yao, Q.,Meng, Y.,Zhou, D.,Bi, L.J.,Rose, J.,Adang, M.J.,Wang, B.C.,Yu, Z.,Sun, M. Crystal structure of Cry51Aa1: A potential novel insecticidal aerolysin-type beta-pore-forming toxin from Bacillus thuringiensis. Biochem.Biophys.Res.Commun., 462:184-189, 2015 Cited by PubMed Abstract: The structures of several Bacillus thuringiensis (Bt) insecticidal crystal proteins have been determined by crystallographic methods and a close relationship has been explicated between specific toxicities and conserved three-dimensional architectures. In this study, as a representative of the coleopteran- and hemipteran-specific Cry51A group, the complete structure of Cry51Aa1 protoxin has been determined by X-ray crystallography at 1.65 Å resolution. This is the first report of a coleopteran-active Bt insecticidal toxin with high structural similarity to the aerolysin-type β-pore forming toxins (β-PFTs). Moreover, study of featured residues and structural elements reveal their possible roles in receptor binding and pore formation events. This study provides new insights into the action of aerolysin-type β-PFTs from a structural perspective, and could be useful for the control of coleopteran and hemipteran insect pests in agricultures. PubMed: 25957471DOI: 10.1016/j.bbrc.2015.04.068 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.65 Å) |
Structure validation
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