4PK7

crystal structure of human Stromal Antigen 2 (SA2) in complex with Sister Chromatid Cohesion protein 1 (Scc1) with bound MES, native proteins

4PK7 の概要

• エントリーDOI: 10.2210/pdb4pk7/pdb
• 分子名称: Cohesin subunit SA-2, Double-strand-break repair protein rad21 homolog, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID (3 entities in total)
• 機能のキーワード: sister chromatid cohesion, cohesin subunits, protein-protein interaction, heat repeat, cell cycle
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 131948.27

構造登録者

Hara, K.,Chen, Z.,Tomchick, D.R.,Yu, H. (登録日: 2014-05-13, 公開日: 2014-09-03, 最終更新日: 2023-09-27)

主引用文献

Hara, K.,Zheng, G.,Qu, Q.,Liu, H.,Ouyang, Z.,Chen, Z.,Tomchick, D.R.,Yu, H.
Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion.
Nat.Struct.Mol.Biol., 21:864-870, 2014

PubMed Abstract: Orderly termination of sister-chromatid cohesion during mitosis is critical for accurate chromosome segregation. During prophase, mitotic kinases phosphorylate cohesin and its protector sororin, triggering Wapl-dependent cohesin release from chromosome arms. The shugoshin (Sgo1)-PP2A complex protects centromeric cohesin until its cleavage by separase at anaphase onset. Here, we report the crystal structure of a human cohesin subcomplex comprising SA2 and Scc1. Multiple HEAT repeats of SA2 form a dragon-shaped structure. Scc1 makes extensive contacts with SA2, with one binding hotspot. Sgo1 and Wapl compete for binding to a conserved site on SA2-Scc1. At this site, mutations of SA2 residues that disrupt Wapl binding bypass the Sgo1 requirement in cohesion protection. Thus, in addition to recruiting PP2A to dephosphorylate cohesin and sororin, Sgo1 physically shields cohesin from Wapl. This unexpected, direct antagonism between Sgo1 and Wapl augments centromeric cohesion protection.

PubMed: 25173175
DOI: 10.1038/nsmb.2880

実験手法

X-RAY DIFFRACTION (2.95 Å)