4PJW
crystal structure of human Stromal Antigen 2 (SA2) in complex with Sister Chromatid Cohesion protein 1 (Scc1), with bound MES
Summary for 4PJW
| Entry DOI | 10.2210/pdb4pjw/pdb |
| Related | 4PJU |
| Descriptor | Cohesin subunit SA-2, Double-strand-break repair protein rad21 homolog, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, ... (4 entities in total) |
| Functional Keywords | sister chromatid cohesion, cohesin subunits, protein-protein interaction, heat repeat, cell cycle |
| Biological source | Homo sapiens (Human) More |
| Total number of polymer chains | 2 |
| Total formula weight | 131871.44 |
| Authors | Hara, K.,Chen, Z.,Tomchick, D.R.,Yu, H. (deposition date: 2014-05-12, release date: 2014-08-27, Last modification date: 2024-11-06) |
| Primary citation | Hara, K.,Zheng, G.,Qu, Q.,Liu, H.,Ouyang, Z.,Chen, Z.,Tomchick, D.R.,Yu, H. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Nat.Struct.Mol.Biol., 21:864-870, 2014 Cited by PubMed Abstract: Orderly termination of sister-chromatid cohesion during mitosis is critical for accurate chromosome segregation. During prophase, mitotic kinases phosphorylate cohesin and its protector sororin, triggering Wapl-dependent cohesin release from chromosome arms. The shugoshin (Sgo1)-PP2A complex protects centromeric cohesin until its cleavage by separase at anaphase onset. Here, we report the crystal structure of a human cohesin subcomplex comprising SA2 and Scc1. Multiple HEAT repeats of SA2 form a dragon-shaped structure. Scc1 makes extensive contacts with SA2, with one binding hotspot. Sgo1 and Wapl compete for binding to a conserved site on SA2-Scc1. At this site, mutations of SA2 residues that disrupt Wapl binding bypass the Sgo1 requirement in cohesion protection. Thus, in addition to recruiting PP2A to dephosphorylate cohesin and sororin, Sgo1 physically shields cohesin from Wapl. This unexpected, direct antagonism between Sgo1 and Wapl augments centromeric cohesion protection. PubMed: 25173175DOI: 10.1038/nsmb.2880 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.85 Å) |
Structure validation
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