4PIP

Engineered EgtD variant EgtD-M252V,E282A in complex with tryptophan and SAH

4PIP の概要

• エントリーDOI: 10.2210/pdb4pip/pdb
• 関連するPDBエントリー: 4PIM 4PIN 4PIO
• 分子名称: Histidine-specific methyltransferase EgtD, TRYPTOPHAN, S-ADENOSYL-L-HOMOCYSTEINE, ... (6 entities in total)
• 機能のキーワード: methyltransferase, ergothioneine, histidine betaine, transferase
• 由来する生物種: Mycobacterium smegmatis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 143208.98

構造登録者

Vit, A.,Seebeck, F.P.,Blankenfeldt, W. (登録日: 2014-05-09, 公開日: 2014-12-03, 最終更新日: 2023-09-27)

主引用文献

Vit, A.,Misson, L.,Blankenfeldt, W.,Seebeck, F.P.
Ergothioneine Biosynthetic Methyltransferase EgtD Reveals the Structural Basis of Aromatic Amino Acid Betaine Biosynthesis.
Chembiochem, 16:119-125, 2015

PubMed Abstract: Ergothioneine is an N-α-trimethyl-2-thiohistidine derivative that occurs in human, plant, fungal, and bacterial cells. Biosynthesis of this redox-active betaine starts with trimethylation of the α-amino group of histidine. The three consecutive methyl transfers are catalyzed by the S-adenosylmethionine-dependent methyltransferase EgtD. Three crystal structures of this enzyme in the absence and in the presence of N-α-dimethylhistidine and S-adenosylhomocysteine implicate a preorganized array of hydrophilic interactions as the determinants for substrate specificity and apparent processivity. We identified two active site mutations that change the substrate specificity of EgtD 10(7)-fold and transform the histidine-methyltransferase into a proficient tryptophan-methyltransferase. Finally, a genomic search for EgtD homologues in fungal genomes revealed tyrosine and tryptophan trimethylation activity as a frequent trait in ascomycetous and basidomycetous fungi.

PubMed: 25404173
DOI: 10.1002/cbic.201402522

実験手法

X-RAY DIFFRACTION (1.8 Å)