4PDK

FadR, Fatty Acid Responsive Transcription Factor from Vibrio cholerae, in Complex with oleoyl-CoA

4PDK の概要

• エントリーDOI: 10.2210/pdb4pdk/pdb
• 関連するPDBエントリー: 4P96 4P9U
• 分子名称: Fatty acid metabolism regulator protein, S-{(3R,5R,9R)-1-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9-trihydroxy-8,8-dimethyl-3,5-dioxido-10,14-dioxo-2,4,6-trioxa-11,15-diaza-3lambda~5~,5lambda~5~-diphosphaheptadecan-17-yl} (9Z)-octadec-9-enethioate (non-preferred name) (2 entities in total)
• 機能のキーワード: transcription regulator, transcription
• 由来する生物種: Vibrio cholerae serotype O1 (ATCC 39315 / El Tor Inaba N16961)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 68197.26

構造登録者

Shi, W.,Kull, F.J. (登録日: 2014-04-19, 公開日: 2015-02-18, 最終更新日: 2023-12-27)

主引用文献

Shi, W.,Kovacikova, G.,Lin, W.,Taylor, R.K.,Skorupski, K.,Kull, F.J.
The 40-residue insertion in Vibrio cholerae FadR facilitates binding of an additional fatty acyl-CoA ligand.
Nat Commun, 6:6032-6032, 2015

PubMed Abstract: FadR is a master regulator of fatty acid metabolism and influences virulence in certain members of Vibrionaceae. Among FadR homologues of the GntR family, the Vibrionaceae protein is unusual in that it contains a C-terminal 40-residue insertion. Here we report the structure of Vibrio cholerae FadR (VcFadR) alone, bound to DNA, and in the presence of a ligand, oleoyl-CoA. Whereas Escherichia coli FadR (EcFadR) contains only one acyl-CoA-binding site in each monomer, crystallographic and calorimetric data indicate that VcFadR has two. One of the binding sites resembles that of EcFadR, whereas the other, comprised residues from the insertion, has not previously been observed. Upon ligand binding, VcFadR undergoes a dramatic conformational change that would more fully disrupt DNA binding than EcFadR. These findings suggest that the ability to bind and respond to an additional ligand allows FadR from Vibrionaceae to function as a more efficient regulator.

PubMed: 25607896
DOI: 10.1038/ncomms7032

実験手法

X-RAY DIFFRACTION (2.8 Å)