4PCF

Structure-based protein engineering of a monomeric triosephosphate isomerase towards changing substrate specificity

Summary for 4PCF

Related5TIM 1TRI 2VEK 2VEL 1AG1 1DKW 1IIG 1ML1 1MSS 2V0T 2V5L 6TIM 2VEM 2VEN 2VEI 3TIM
DescriptorMa18-TIM (2 entities in total)
Functional Keywordstriosephosphate isomerase, tim barrel, protein engineering, substrate specificity, isomerase
Biological sourceTrypanosoma brucei brucei
Total number of polymer chains3
Total molecular weight77509.8
Authors
Krause, M.,Neubauer, P.,Wierenga, R.K. (deposition date: 2014-04-15, release date: 2015-04-22, Last modification date: 2016-06-29)
Primary citation
Krause, M.,Kiema, T.R.,Neubauer, P.,Wierenga, R.K.
Crystal structures of two monomeric triosephosphate isomerase variants identified via a directed-evolution protocol selecting for L-arabinose isomerase activity.
Acta Crystallogr.,Sect.F, 72:490-499, 2016
PubMed: 27303904 (PDB entries with the same primary citation)
DOI: 10.1107/S2053230X16007548
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.71 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.2828 1.1% 5.7% 2.3%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload
171916
PDB entries from 2020-12-02