4P8S

Crystal structure of Nogo-receptor-2

4P8S の概要

• エントリーDOI: 10.2210/pdb4p8s/pdb
• 関連するPDBエントリー: 1OZN 4P91
• 分子名称: Reticulon-4 receptor-like 2, 2-acetamido-2-deoxy-alpha-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: nogo receptor, myelin associated glycoprotein, membrane protein
• 由来する生物種: Rattus norvegicus (Rat)
• 細胞内の位置: Cell membrane ; Lipid-anchor, GPI-anchor : Q80WD1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 33009.48

構造登録者

Semavina, M.,Saha, N.,Kolev, M.V.,Giger, R.J.,Himanen, J.P.,Nikolov, D.B. (登録日: 2014-04-01, 公開日: 2014-04-09, 最終更新日: 2024-10-16)

主引用文献

Semavina, M.,Saha, N.,Kolev, M.V.,Goldgur, Y.,Giger, R.J.,Himanen, J.P.,Nikolov, D.B.
Crystal structure of the Nogo-receptor-2.
Protein Sci., :684-689, 2011

PubMed Abstract: The inhibition of axon regeneration upon mechanical injury is dependent on interactions between Nogo receptors (NgRs) and their myelin-derived ligands. NgRs are composed of a leucine-rich repeat (LRR) region, thought to be structurally similar among the different isoforms of the receptor, and a divergent "stalk" region. It has been shown by others that the LRR and stalk regions of NgR1 and NgR2 have distinct roles in conferring binding affinity to the myelin associated glycoprotein (MAG) in vivo. Here, we show that purified recombinant full length NgR1 and NgR2 maintain significantly higher binding affinity for purified MAG as compared to the isolated LRR region of either NgR1 or NgR2. We also present the crystal structure of the LRR and part of the stalk regions of NgR2 and compare it to the previously reported NgR1 structure with respect to the distinct signaling properties of the two receptor isoforms.

PubMed: 21308849
DOI: 10.1002/pro.597

実験手法

X-RAY DIFFRACTION (1.8 Å)