4P69

Acek (D477A) ICDH complex

4P69 の概要

• エントリーDOI: 10.2210/pdb4p69/pdb
• 分子名称: Isocitrate dehydrogenase kinase/phosphatase, Isocitrate dehydrogenase [NADP], ADENOSINE MONOPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: transferase, hydrolase-oxidoreductase complex, hydrolase/oxidoreductase
• 由来する生物種: Escherichia coli O157:H7; 詳細
• 細胞内の位置: Cytoplasm : Q8X607
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 225897.55

構造登録者

Jimin, Z.,Nan, W.,Shu, W.,Zongchao, J. (登録日: 2014-03-22, 公開日: 2014-10-08, 最終更新日: 2023-12-27)

主引用文献

Wang, S.,Shen, Q.,Chen, G.,Zheng, J.,Tan, H.,Jia, Z.
The phosphatase mechanism of bifunctional kinase/phosphatase AceK.
Chem. Commun. (Camb.), 50:14117-14120, 2014

PubMed Abstract: We have revealed that bifunctional AceK kinase/phosphatase utilizes a stepwise addition-elimination mechanism in its dephosphorylation reaction. This work explains how AceK enables opposite kinase and phosphatase activities with Asp477 and a single Mg(2+) ion.

PubMed: 25272278
DOI: 10.1039/c4cc05375c

実験手法

X-RAY DIFFRACTION (3.3 Å)