4P2L
Quiescin Sulfhydryl Oxidase from Rattus norvegicus
Summary for 4P2L
Entry DOI | 10.2210/pdb4p2l/pdb |
Related | 3LLK 3Q6O 3QCP 3T58 |
Descriptor | Sulfhydryl oxidase 1, FLAVIN-ADENINE DINUCLEOTIDE (3 entities in total) |
Functional Keywords | disulfide formation, enzyme intermediate, thioredoxin fold, erv fold, oxidoreductase |
Biological source | Rattus norvegicus (Rat) |
Total number of polymer chains | 2 |
Total formula weight | 115176.42 |
Authors | |
Primary citation | Gat, Y.,Vardi-Kilshtain, A.,Grossman, I.,Major, D.T.,Fass, D. Enzyme structure captures four cysteines aligned for disulfide relay. Protein Sci., 23:1102-1112, 2014 Cited by PubMed Abstract: Thioredoxin superfamily proteins introduce disulfide bonds into substrates, catalyze the removal of disulfides, and operate in electron relays. These functions rely on one or more dithiol/disulfide exchange reactions. The flavoenzyme quiescin sulfhydryl oxidase (QSOX), a catalyst of disulfide bond formation with an interdomain electron transfer step in its catalytic cycle, provides a unique opportunity for exploring the structural environment of enzymatic dithiol/disulfide exchange. Wild-type Rattus norvegicus QSOX1 (RnQSOX1) was crystallized in a conformation that juxtaposes the two redox-active di-cysteine motifs in the enzyme, presenting the entire electron-transfer pathway and proton-transfer participants in their native configurations. As such a state cannot generally be enriched and stabilized for analysis, RnQSOX1 gives unprecedented insight into the functional group environments of the four cysteines involved in dithiol/disulfide exchange and provides the framework for analysis of the energetics of electron transfer in the presence of the bound flavin adenine dinucleotide cofactor. Hybrid quantum mechanics/molecular mechanics (QM/MM) free energy simulations based on the X-ray crystal structure suggest that formation of the interdomain disulfide intermediate is highly favorable and secures the flexible enzyme in a state from which further electron transfer via the flavin can occur. PubMed: 24888638DOI: 10.1002/pro.2496 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.9 Å) |
Structure validation
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