4OYM

Human solAC Complexed with (4-Amino-furazan-3-yl)-(3-methoxy-phenyl)-methanone

4OYM の概要

• エントリーDOI: 10.2210/pdb4oym/pdb
• 関連するPDBエントリー: 4OYA 4OYB 4OYI 4OYO 4OYP 4OYW 4OYX 4OYZ 4OZ2 4OZ3
• 分子名称: Adenylate cyclase type 10, (4-azanyl-1,2,5-oxadiazol-3-yl)-(3-methoxyphenyl)methanone, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: lyase
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Cell membrane; Peripheral membrane protein; Cytoplasmic side: Q96PN6
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 53870.22

構造登録者

Vinkovic, M. (登録日: 2014-02-12, 公開日: 2014-04-02, 最終更新日: 2024-10-23)

主引用文献

Saalau-Bethell, S.M.,Berdini, V.,Cleasby, A.,Congreve, M.,Coyle, J.E.,Lock, V.,Murray, C.W.,O'Brien, M.A.,Rich, S.J.,Sambrook, T.,Vinkovic, M.,Yon, J.R.,Jhoti, H.
Crystal structure of human soluble adenylate cyclase reveals a distinct, highly flexible allosteric bicarbonate binding pocket.
Chemmedchem, 9:823-832, 2014

PubMed Abstract: Soluble adenylate cyclases catalyse the synthesis of the second messenger cAMP through the cyclisation of ATP and are the only known enzymes to be directly activated by bicarbonate. Here, we report the first crystal structure of the human enzyme that reveals a pseudosymmetrical arrangement of two catalytic domains to produce a single competent active site and a novel discrete bicarbonate binding pocket. Crystal structures of the apo protein, the protein in complex with α,β-methylene adenosine 5'-triphosphate (AMPCPP) and calcium, with the allosteric activator bicarbonate, and also with a number of inhibitors identified using fragment screening, all show a flexible active site that undergoes significant conformational changes on binding of ligands. The resulting nanomolar-potent inhibitors that were developed bind at both the substrate binding pocket and the allosteric site, and can be used as chemical probes to further elucidate the function of this protein.

PubMed: 24616449
DOI: 10.1002/cmdc.201300480

実験手法

X-RAY DIFFRACTION (1.7 Å)