4OWW

Structural basis of SOSS1 in complex with a 35nt ssDNA

Summary for 4OWW

• Entry DOI: 10.2210/pdb4oww/pdb
• Descriptor: DNA (5'-D(P*TP*TP*TP*TP*TP*T)-3'), Integrator complex subunit 3, SOSS complex subunit B1, ... (5 entities in total)
• Functional Keywords: soss1 complex, dna double-strand breaks, homologous recombination, ssdna-binding protein, dna binding protein
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 4
• Total formula weight: 100471.68

Authors

Ren, W.,Sun, Q.,Tang, X.,Song, H. (deposition date: 2014-02-04, release date: 2014-04-16, Last modification date: 2023-12-27)

Primary citation

Ren, W.,Chen, H.,Sun, Q.,Tang, X.,Lim, S.C.,Huang, J.,Song, H.
Structural Basis of SOSS1 Complex Assembly and Recognition of ssDNA.
Cell Rep, 6:982-991, 2014

PubMed Abstract: The SOSS1 complex comprising SOSSA, SOSSB1, and SOSSC senses single-stranded DNA (ssDNA) and promotes repair of DNA double-strand breaks (DSBs). But how SOSS1 is assembled and recognizes ssDNA remains elusive. The crystal structure of the N-terminal half of SOSSA (SOSSAN) in complex with SOSSB1 and SOSSC showed that SOSSAN serves as a scaffold to bind both SOSSB1 and SOSSC for assembly of the SOSS1 complex. The structures of SOSSAN/B1 in complex with a 12 nt ssDNA and SOSSAN/B1/C in complex with a 35 nt ssDNA showed that SOSSB1 interacts with both SOSSAN and ssDNA via two distinct surfaces. Recognition of ssDNA with a length of up to nine nucleotides is mediated solely by SOSSB1, whereas neither SOSSC nor SOSSAN are critical for ssDNA binding. These results reveal the structural basis of SOSS1 assembly and provide a framework for further study of the mechanism governing longer ssDNA recognition by the SOSS1 complex during DSB repair.

PubMed: 24630995
DOI: 10.1016/j.celrep.2014.02.020

Experimental method

X-RAY DIFFRACTION (2.3 Å)