4OWH

PtBr6 binding to HEWL

Summary for 4OWH

• Entry DOI: 10.2210/pdb4owh/pdb
• Descriptor: Lysozyme C, HEXABROMOPLATINATE(IV), BROMIDE ION, ... (7 entities in total)
• Functional Keywords: hydrolase
• Biological source: Gallus gallus
• Cellular location: Secreted: P00698
• Total number of polymer chains: 1
• Total formula weight: 16300.13

Authors

Tanley, S.W.M.,Starkey, V.L.,Lamplough, L.,Kaenket, S.,Helliwell, J.R. (deposition date: 2014-02-02, release date: 2014-09-24, Last modification date: 2024-11-13)

Primary citation

Tanley, S.W.,Starkey, L.V.,Lamplough, L.,Kaenket, S.,Helliwell, J.R.
The binding of platinum hexahalides (Cl, Br and I) to hen egg-white lysozyme and the chemical transformation of the PtI6 octahedral complex to a PtI3 moiety bound to His15.
Acta Crystallogr.,Sect.F, 70:1132-1134, 2014

PubMed Abstract: This study examines the binding and chemical stability of the platinum hexahalides K2PtCl6, K2PtBr6 and K2PtI6 when soaked into pre-grown hen egg-white lysozyme (HEWL) crystals as the protein host. Direct comparison of the iodo complex with the chloro and bromo complexes shows that the iodo complex is partly chemically transformed to a square-planar PtI3 complex bound to the N(δ) atom of His15, a chemical behaviour that is not exhibited by the chloro or bromo complexes. Each complex does, however, bind to HEWL in its octahedral form either at one site (PtI6) or at two sites (PtBr6 and PtCl6). As heavy-atom derivatives of a protein, the octahedral shape of the hexahalides could be helpful in cases of difficult-to-interpret electron-density maps as they would be recognisable 'objects'.

PubMed: 25195880
DOI: 10.1107/S2053230X14014009

Experimental method

X-RAY DIFFRACTION (1.484 Å)