4OW3

Thermolysin structure determined by free-electron laser

Summary for 4OW3

DescriptorThermolysin, ZINC ION, CALCIUM ION, ... (4 entities in total)
Functional Keywordsthermolysin, protease, hydrolase
Biological sourceBacillus thermoproteolyticus
Cellular locationSecreted P00800
Total number of polymer chains1
Total molecular weight34586.06
Authors
Primary citation
Hattne, J.,Echols, N.,Tran, R.,Kern, J.,Gildea, R.J.,Brewster, A.S.,Alonso-Mori, R.,Glockner, C.,Hellmich, J.,Laksmono, H.,Sierra, R.G.,Lassalle-Kaiser, B.,Lampe, A.,Han, G.,Gul, S.,DiFiore, D.,Milathianaki, D.,Fry, A.R.,Miahnahri, A.,White, W.E.,Schafer, D.W.,Seibert, M.M.,Koglin, J.E.,Sokaras, D.,Weng, T.C.,Sellberg, J.,Latimer, M.J.,Glatzel, P.,Zwart, P.H.,Grosse-Kunstleve, R.W.,Bogan, M.J.,Messerschmidt, M.,Williams, G.J.,Boutet, S.,Messinger, J.,Zouni, A.,Yano, J.,Bergmann, U.,Yachandra, V.K.,Adams, P.D.,Sauter, N.K.
Accurate macromolecular structures using minimal measurements from X-ray free-electron lasers.
Nat.Methods, 11:545-548, 2014
PubMed: 24633409 (PDB entries with the same primary citation)
DOI: 10.1038/nmeth.2887
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.1 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.265100.8%0MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 4ow3
no rotation
Molmil generated image of 4ow3
rotated about x axis by 90°
Molmil generated image of 4ow3
rotated about y axis by 90°