4OSK

Crystal structure of TAL effector reveals the recognition between asparagine and guanine

Summary for 4OSK

• Entry DOI: 10.2210/pdb4osk/pdb
• Related: 4OSH 4OSI 4OSJ 4OSL 4OSM 4OSQ 4OSR 4OSS 4OST 4OSV 4OSW 4OSZ 4OT0 4OT3 4OTO
• Descriptor: Hax3, DNA (5'-D(*TP*GP*TP*CP*CP*AP*AP*CP*TP*AP*CP*TP*AP*GP*AP*CP*T)-3'), DNA (5'-D(*AP*GP*TP*CP*TP*AP*GP*TP*AP*GP*TP*TP*GP*GP*AP*CP*A)-3'), ... (4 entities in total)
• Functional Keywords: dna binding protein, dna, dna binding protein-dna complex, dna binding protein/dna
• Biological source: Xanthomonas campestris pv. armoraciae
• Total number of polymer chains: 6
• Total formula weight: 124524.76

Authors

Deng, D.,Wu, J.P.,Yan, C.Y.,Pan, X.J.,Yan, N. (deposition date: 2014-02-13, release date: 2014-05-28, Last modification date: 2023-11-08)

Primary citation

Deng, D.,Yan, C.Y.,Wu, J.P.,Pan, X.J.,Yan, N.
Revisiting the TALE repeat
Protein Cell, 5:297-306, 2014

PubMed Abstract: Transcription activator-like (TAL) effectors specifically bind to double stranded (ds) DNA through a central domain of tandem repeats. Each TAL effector (TALE) repeat comprises 33-35 amino acids and recognizes one specific DNA base through a highly variable residue at a fixed position in the repeat. Structural studies have revealed the molecular basis of DNA recognition by TALE repeats. Examination of the overall structure reveals that the basic building block of TALE protein, namely a helical hairpin, is one-helix shifted from the previously defined TALE motif. Here we wish to suggest a structure-based re-demarcation of the TALE repeat which starts with the residues that bind to the DNA backbone phosphate and concludes with the base-recognition hyper-variable residue. This new numbering system is consistent with the α-solenoid superfamily to which TALE belongs, and reflects the structural integrity of TAL effectors. In addition, it confers integral number of TALE repeats that matches the number of bound DNA bases. We then present fifteen crystal structures of engineered dHax3 variants in complex with target DNA molecules, which elucidate the structural basis for the recognition of bases adenine (A) and guanine (G) by reported or uncharacterized TALE codes. Finally, we analyzed the sequence-structure correlation of the amino acid residues within a TALE repeat. The structural analyses reported here may advance the mechanistic understanding of TALE proteins and facilitate the design of TALEN with improved affinity and specificity.

PubMed: 24622844
DOI: 10.1007/s13238-014-0035-2

Experimental method

X-RAY DIFFRACTION (2.398 Å)