4OOR

Ancestral Steroid Receptor 2 DNA binding domain in complex with a steroid response element

Summary for 4OOR

• Entry DOI: 10.2210/pdb4oor/pdb
• Related: 4OLN 4OND
• Descriptor: Ancestral Steroid Receptor 2 DNA binding domain, 5'-D(*CP*CP*AP*GP*AP*AP*CP*AP*GP*AP*GP*TP*GP*TP*TP*CP*TP*G)-3', 5'-D(*TP*CP*AP*GP*AP*AP*CP*AP*CP*TP*CP*TP*GP*TP*TP*CP*TP*G)-3', ... (5 entities in total)
• Functional Keywords: nuclear receptor zinc finger, steroid receptor, transcription factor, dna, coregulators, transcription-dna complex, transcription/dna
• Biological source: synthetic construct
• Total number of polymer chains: 8
• Total formula weight: 58684.75

Authors

Ortlund, E.O.,Murphy, M.N. (deposition date: 2014-02-03, release date: 2014-10-29, Last modification date: 2024-02-28)

Primary citation

McKeown, A.N.,Bridgham, J.T.,Anderson, D.W.,Murphy, M.N.,Ortlund, E.A.,Thornton, J.W.
Evolution of DNA specificity in a transcription factor family produced a new gene regulatory module.
Cell(Cambridge,Mass.), 159:58-68, 2014

PubMed Abstract: Complex gene regulatory networks require transcription factors (TFs) to bind distinct DNA sequences. To understand how novel TF specificity evolves, we combined phylogenetic, biochemical, and biophysical approaches to interrogate how DNA recognition diversified in the steroid hormone receptor (SR) family. After duplication of the ancestral SR, three mutations in one copy radically weakened binding to the ancestral estrogen response element (ERE) and improved binding to a new set of DNA sequences (steroid response elements, SREs). They did so by establishing unfavorable interactions with ERE and abolishing unfavorable interactions with SRE; also required were numerous permissive substitutions, which nonspecifically improved cooperativity and affinity of DNA binding. Our findings indicate that negative determinants of binding play key roles in TFs' DNA selectivity and-with our prior work on the evolution of SR ligand specificity during the same interval-show how a specific new gene regulatory module evolved without interfering with the integrity of the ancestral module.

PubMed: 25259920
DOI: 10.1016/j.cell.2014.09.003

Experimental method

X-RAY DIFFRACTION (2.701 Å)