4OKL

Crystal Structure of d(CCCCGGTACCGGGG)2 at 1.65 Angstrom

Summary for 4OKL

• Entry DOI: 10.2210/pdb4okl/pdb
• Related: 3QK4 3V9D
• Descriptor: 14 mer A-DNA (2 entities in total)
• Functional Keywords: a-dna, tetra-decanucleotide, crystal packing, right handed, double helix, dna
• Total number of polymer chains: 2
• Total formula weight: 8565.54

Authors

Anna, V.,Monica, P.,Mandal, P.K.,Gautham, N. (deposition date: 2014-01-22, release date: 2014-02-19, Last modification date: 2023-09-20)

Primary citation

Purushothaman, M.,Varghese, A.,Mandal, P.K.,Gautham, N.
Structure of d(CCCCGGTACCGGGG)2 at 1.65 angstrom resolution.
Acta Crystallogr F Struct Biol Commun, 70:860-865, 2014

PubMed Abstract: The crystal structure of the tetradecanucleotide d(CCCCGGTACCGGGG)2 has previously been reported as an A-type double helix at a resolution of 2.5 Å in space group P41. Here, the structure of this sequence was determined at a significantly higher resolution of 1.65 Å in space group P4₁2₁2. The differences in crystal packing between the former and latter are described. The crystallographic asymmetric unit consists of one tetradecanucleotide duplex that spans more than one full turn of the A-helix. This structure allowed the unambiguous identification of solvent interactions.

PubMed: 25005078
DOI: 10.1107/S2053230X1401084X

Experimental method

X-RAY DIFFRACTION (1.65 Å)