4OJI
Crystal Structure of Twister Ribozyme
Summary for 4OJI
| Entry DOI | 10.2210/pdb4oji/pdb |
| Descriptor | RNA (52-MER), MAGNESIUM ION (3 entities in total) |
| Functional Keywords | pseudoknot, self-cleavage, rna |
| Total number of polymer chains | 1 |
| Total formula weight | 17579.03 |
| Authors | Liu, Y.,Wilson, T.J.,McPhee, S.A.,Lilley, D.M.J. (deposition date: 2014-01-21, release date: 2014-07-23, Last modification date: 2024-02-28) |
| Primary citation | Liu, Y.,Wilson, T.J.,McPhee, S.A.,Lilley, D.M. Crystal structure and mechanistic investigation of the twister ribozyme. Nat.Chem.Biol., 10:739-744, 2014 Cited by PubMed Abstract: We present a crystal structure at 2.3-Å resolution of the recently described nucleolytic ribozyme twister. The RNA adopts a previously uncharacterized compact fold based on a double-pseudoknot structure, with the active site at its center. Eight highly conserved nucleobases stabilize the core of the ribozyme through the formation of one Watson-Crick and three noncanonical base pairs, and the highly conserved adenine 3' of the scissile phosphate is bound in the major groove of an adjacent pseudoknot. A strongly conserved guanine nucleobase directs its Watson-Crick edge toward the scissile phosphate in the crystal structure, and mechanistic evidence supports a role for this guanine as either a general base or acid in a concerted, general acid-base-catalyzed cleavage reaction. PubMed: 25038788DOI: 10.1038/nchembio.1587 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.3 Å) |
Structure validation
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