4OJF

Humanised 3D6 Fab complexed to amyloid beta 1-8

Summary for 4OJF

• Entry DOI: 10.2210/pdb4ojf/pdb
• Related: 4HIX
• Descriptor: Humanised 3D6 Fab Heavy Chain, Humanised 3D6 Fab Light Chain, Amyloid beta A4 protein, ... (4 entities in total)
• Functional Keywords: amyloid, bapineuzumab, 3d6, alzheimer's disease, immunoglobulin, immunotherapy, amyloid beta, immune system
• Biological source: Mus musculus (mouse); More
• Cellular location: Membrane; Single-pass type I membrane protein: P05067
• Total number of polymer chains: 3
• Total formula weight: 49274.90

Authors

Miles, L.A.,Crespi, G.A.N.,Parker, M.W. (deposition date: 2014-01-21, release date: 2015-01-28, Last modification date: 2024-10-16)

Primary citation

Crespi, G.A.N.,Ascher, D.B.,Parker, M.W.,Miles, L.A.
Crystallization and preliminary X-ray diffraction analysis of the Fab portion of the Alzheimer's disease immunotherapy candidate bapineuzumab complexed with amyloid-beta
ACTA CRYSTALLOGR.,SECT.F, 70:374-377, 2014

PubMed Abstract: Bapineuzumab (AAB-001) and its derivative (AAB-003) are humanized versions of the anti-Aβ murine antibody 3D6 and are immunotherapy candidates in Alzheimer's disease. The common Fab fragment of these immunotherapies has been expressed, purified and crystallized in complex with β-amyloid peptides (residues 1-8 and 1-28). Diffraction data at high resolution were acquired from crystals of Fab-Aβ8 (2.0 Å) and Fab-Aβ28 (2.2 Å) complexes at the Australian Synchrotron. Both crystal forms belonged to the primitive orthorhombic space group P21221.

PubMed: 24598931
DOI: 10.1107/S2053230X14001642

Experimental method

X-RAY DIFFRACTION (1.998 Å)