Crystal structure of Thermus thermophilus pre-insertion substrate complex for de novo transcription initiation

Summary for 4OIO

Related4OIN 4OIP 4OIQ 4OIR
DescriptorDNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (12 entities in total)
Functional Keywordsde novo transcription initiation, substrate complex, transcription initiation, i site, i+1 site, rna polymerase, dna/rna/ntp binding, polymerization of ribonucleotides, nucleoid, transcription, transferase
Biological sourceThermus thermophilus
Cellular locationCytoplasm (By similarity) Q5SKW1
Total number of polymer chains8
Total molecular weight444918.37
Zhang, Y.,Ebright, R.H.,Arnold, E. (deposition date: 2014-01-20, release date: 2014-05-07)
Primary citation
Zhang, Y.,Degen, D.,Ho, M.X.,Sineva, E.,Ebright, K.Y.,Ebright, Y.W.,Mekler, V.,Vahedian-Movahed, H.,Feng, Y.,Yin, R.,Tuske, S.,Irschik, H.,Jansen, R.,Maffioli, S.,Donadio, S.,Arnold, E.,Ebright, R.H.
GE23077 binds to the RNA polymerase 'i' and 'i+1' sites and prevents the binding of initiating nucleotides.
Elife, 3:e02450-e02450, 2014
PubMed: 24755292 (PDB entries with the same primary citation)
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.25680.1%3.8%0.7%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

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