4OIO

Crystal structure of Thermus thermophilus pre-insertion substrate complex for de novo transcription initiation

Summary for 4OIO

Related4OIN 4OIP 4OIQ 4OIR
DescriptorDNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (12 entities in total)
Functional Keywordsde novo transcription initiation, substrate complex, transcription initiation, i site, i+1 site, rna polymerase, dna/rna/ntp binding, polymerization of ribonucleotides, nucleoid, transcription, transferase
Biological sourceThermus thermophilus
Cellular locationCytoplasm (By similarity) Q5SKW1
Total number of polymer chains8
Total molecular weight444918.37
Authors
Zhang, Y.,Ebright, R.H.,Arnold, E. (deposition date: 2014-01-20, release date: 2014-05-07)
Primary citation
Zhang, Y.,Degen, D.,Ho, M.X.,Sineva, E.,Ebright, K.Y.,Ebright, Y.W.,Mekler, V.,Vahedian-Movahed, H.,Feng, Y.,Yin, R.,Tuske, S.,Irschik, H.,Jansen, R.,Maffioli, S.,Donadio, S.,Arnold, E.,Ebright, R.H.
GE23077 binds to the RNA polymerase 'i' and 'i+1' sites and prevents the binding of initiating nucleotides.
Elife, 3:e02450-e02450, 2014
PubMed: 24755292 (PDB entries with the same primary citation)
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3.1 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.25680.1%3.8%0.7%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

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