4OBV

Ruminococcus gnavus tryptophan decarboxylase RUMGNA_01526 (alpha-FMT)

4OBV の概要

• エントリーDOI: 10.2210/pdb4obv/pdb
• 関連するPDBエントリー: 4OBU
• 分子名称: Pyridoxal-dependent decarboxylase domain protein, alpha-(fluoromethyl)-D-tryptophan, {5-hydroxy-4-[(1E)-4-(1H-indol-3-yl)-3-oxobut-1-en-1-yl]-6-methylpyridin-3-yl}methyl dihydrogen phosphate (3 entities in total)
• 機能のキーワード: type 1 plp-dependent, decarboxylase, lyase
• 由来する生物種: Ruminococcus gnavus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 222652.80

構造登録者

Fraser, J.S.,Van Benschoten, A.H. (登録日: 2014-01-07, 公開日: 2014-10-29, 最終更新日: 2024-02-28)

主引用文献

Williams, B.B.,Van Benschoten, A.H.,Cimermancic, P.,Donia, M.S.,Zimmermann, M.,Taketani, M.,Ishihara, A.,Kashyap, P.C.,Fraser, J.S.,Fischbach, M.A.
Discovery and Characterization of Gut Microbiota Decarboxylases that Can Produce the Neurotransmitter Tryptamine.
Cell Host Microbe, 16:495-503, 2014

PubMed Abstract: Several recent studies describe the influence of the gut microbiota on host brain and behavior. However, the mechanisms responsible for microbiota-nervous system interactions are largely unknown. Using a combination of genetics, biochemistry, and crystallography, we identify and characterize two phylogenetically distinct enzymes found in the human microbiome that decarboxylate tryptophan to form the β-arylamine neurotransmitter tryptamine. Although this enzymatic activity is exceedingly rare among bacteria more broadly, analysis of the Human Microbiome Project data demonstrate that at least 10% of the human population harbors at least one bacterium encoding a tryptophan decarboxylase in their gut community. Our results uncover a previously unrecognized enzymatic activity that can give rise to host-modulatory compounds and suggests a potential direct mechanism by which gut microbiota can influence host physiology, including behavior.

PubMed: 25263219
DOI: 10.1016/j.chom.2014.09.001

実験手法

X-RAY DIFFRACTION (2.84 Å)