4OBC

Crystal structure of HCV polymerase NS5b genotype 2a JFH-1 isolate with the S15G, C223H, V321I resistance mutations against the guanosine analog GS-0938 (PSI-3529238)

Summary for 4OBC

• Entry DOI: 10.2210/pdb4obc/pdb
• Related: 4E76 4E78 4E7A
• Descriptor: RNA-directed RNA polymerase, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, GLYCEROL, ... (6 entities in total)
• Functional Keywords: hepatitis, hcv, viral polymerase, rna-dependent-rna-polymerase, rdrp, resistance, nucleotide analog inhibitor, viral protein, transferase
• Biological source: Hepatitis C virus JFH-1 (HCV)
• Cellular location: Core protein p21: Host endoplasmic reticulum membrane ; Single-pass membrane protein . Core protein p19: Virion . Envelope glycoprotein E1: Virion membrane ; Single-pass type I membrane protein . Envelope glycoprotein E2: Virion membrane ; Single-pass type I membrane protein . p7: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Protease NS2-3: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Serine protease NS3: Host endoplasmic reticulum membrane ; Peripheral membrane protein . Non-structural protein 4A: Host endoplasmic reticulum membrane ; Single-pass type I membrane protein . Non-structural protein 4B: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Non-structural protein 5A: Host endoplasmic reticulum membrane ; Peripheral membrane protein . RNA-directed RNA polymerase: Host endoplasmic reticulum membrane ; Single-pass type I membrane protein : Q99IB8
• Total number of polymer chains: 1
• Total formula weight: 65500.59

Authors

Edwards, T.E.,Abendroth, J.,Appleby, T.C. (deposition date: 2014-01-07, release date: 2014-09-10, Last modification date: 2023-09-20)

• Primary citation: Lam, A.M.,Edwards, T.E.,Mosley, R.T.,Murakami, E.,Bansal, S.,Lugo, C.,Bao, H.,Otto, M.J.,Sofia, M.J.,Furman, P.A.; Molecular and Structural Basis for the Roles of Hepatitis C Virus Polymerase NS5B Amino Acids 15, 223, and 321 in Viral Replication and Drug Resistance.; Antimicrob.Agents Chemother., 58:6861-6869, 2014; PubMed: 25182647; DOI: 10.1128/AAC.03847-14

Experimental method

X-RAY DIFFRACTION (2.5 Å)