4OBC
Crystal structure of HCV polymerase NS5b genotype 2a JFH-1 isolate with the S15G, C223H, V321I resistance mutations against the guanosine analog GS-0938 (PSI-3529238)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | RNA-directed RNA polymerase | polymer | 580 | 64819.4 | 1 | UniProt (Q99IB8) Pfam (PF00998) In PDB | Hepatitis C virus JFH-1 (HCV) | NS5B, p68 |
2 | A | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
3 | A | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
4 | A | 1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE | non-polymer | 266.3 | 1 | Chemie (PG6) | |||
5 | A | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
6 | water | water | 18.0 | 206 | Chemie (HOH) |
Sequence modifications
A: 1 - 570 (UniProt: Q99IB8)
PDB | External Database | Details |
---|---|---|
Met -1 | - | expression tag |
Ser 0 | - | expression tag |
Gly 15 | Ser 2457 | engineered mutation |
Gln 86 | Glu 2528 | engineered mutation |
Gln 87 | Glu 2529 | engineered mutation |
His 223 | Cys 2665 | engineered mutation |
Ile 321 | Val 2763 | engineered mutation |
Leu 571 | - | expression tag |
Glu 572 | - | expression tag |
His 573 | - | expression tag |
His 574 | - | expression tag |
His 575 | - | expression tag |
His 576 | - | expression tag |
His 577 | - | expression tag |
His 578 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 64819.4 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 681.2 | |
All* | Total formula weight | 65500.6 |