4O9R

Human Smoothened Receptor structure in complex with cyclopamine

4O9R の概要

• エントリーDOI: 10.2210/pdb4o9r/pdb
• 分子名称: Smoothened homolog/Soluble cytochrome b562 chimeric protein, Cyclopamine (2 entities in total)
• 機能のキーワード: serial femtosecond crystallography, human smo receptor, cyclopamine, novel protein engineering, gpcr network, psi-biology, free electron laser, structural genomics, membrane protein, xfel, lcp, room temperature structure, gpcr, membrane, signaling protein
• 由来する生物種: Homo sapiens; 詳細
• 細胞内の位置: Membrane ; Multi-pass membrane protein : Q99835
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 52806.82

構造登録者

Wang, C.,Weierstall, U.,James, D.,White, T.A.,Wang, D.,Liu, W.,Spence, J.C.H.,Doak, R.B.,Nelson, G.,Fromme, P.,Fromme, R.,Grotjohann, I.,Kupitz, C.,Zatsepin, N.A.,Liu, H.,Basu, S.,Wacker, D.,Han, G.W.,Katritch, V.,Boutet, S.,Messerschmidt, M.,Willams, G.J.,Koglin, J.E.,Seibert, M.M.,Klinker, M.,Gati, C.,Shoeman, R.L.,Barty, A.,Chapman, H.N.,Kirian, R.A.,Beyerlein, K.R.,Stevens, R.C.,Li, D.,Shah, S.T.A.,Howe, N.,Caffrey, M.,Cherezov, V.,GPCR Network (GPCR) (登録日: 2014-01-02, 公開日: 2014-03-05, 最終更新日: 2024-10-16)

主引用文献

Weierstall, U.,James, D.,Wang, C.,White, T.A.,Wang, D.,Liu, W.,Spence, J.C.,Bruce Doak, R.,Nelson, G.,Fromme, P.,Fromme, R.,Grotjohann, I.,Kupitz, C.,Zatsepin, N.A.,Liu, H.,Basu, S.,Wacker, D.,Han, G.W.,Katritch, V.,Boutet, S.,Messerschmidt, M.,Williams, G.J.,Koglin, J.E.,Marvin Seibert, M.,Klinker, M.,Gati, C.,Shoeman, R.L.,Barty, A.,Chapman, H.N.,Kirian, R.A.,Beyerlein, K.R.,Stevens, R.C.,Li, D.,Shah, S.T.,Howe, N.,Caffrey, M.,Cherezov, V.
Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography.
Nat Commun, 5:3309-3309, 2014

PubMed Abstract: Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.

PubMed: 24525480
DOI: 10.1038/ncomms4309

実験手法

X-RAY DIFFRACTION (3.204 Å)