4O9M

Human DNA polymerase beta complexed with adenylated tetrahydrofuran (abasic site) containing DNA

4O9M の概要

• エントリーDOI: 10.2210/pdb4o9m/pdb
• 分子名称: DNA (5'-D(*CP*CP*GP*AP*CP*AP*GP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3'), DNA (5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*CP*C)-3'), (5'-D(P*GP*TP*CP*GP*G)-3');, ... (7 entities in total)
• 機能のキーワード: lyase, aprataxin, adenylated tetrahydrofuran, transferase-dna complex, transferase/dna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: P06746
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 48554.35

構造登録者

Caglayan, M.,Batra, V.K.,Sassa, A.,Prasad, R.,Wilson, S.H. (登録日: 2014-01-02, 公開日: 2014-04-30, 最終更新日: 2023-09-20)

主引用文献

Caglayan, M.,Batra, V.K.,Sassa, A.,Prasad, R.,Wilson, S.H.
Role of polymerase beta in complementing aprataxin deficiency during abasic-site base excision repair.
Nat.Struct.Mol.Biol., 21:497-499, 2014

PubMed Abstract: DNA polymerase β (pol β) lyase removal of 5'-deoxyribose phosphate (5'-dRP) from base excision repair (BER) intermediates is critical in mammalian BER involving the abasic site. We found that pol β also removes 5'-adenylated dRP from BER intermediates after abortive ligation. The crystal structure of a human pol β-DNA complex showed the 5'-AMP-dRP group positioned in the lyase active site. Pol β expression rescued methyl methanesulfonate sensitivity in aprataxin (hnt3)- and FEN1 (rad27)-deficient yeast.

PubMed: 24777061
DOI: 10.1038/nsmb.2818

実験手法

X-RAY DIFFRACTION (2.295 Å)