4NZU

Crystal structure of the primary monoclonal antibody 13PL Fab' from a multiple myeloma patient

Summary for 4NZU

• Entry DOI: 10.2210/pdb4nzu/pdb
• Related: 4NZR 4NZT
• Descriptor: 13PL heavy chain, 13PL light chain, GLYCEROL, ... (5 entities in total)
• Functional Keywords: antibody fab, multiple myeloma, primary antibody, immune system
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 2
• Total formula weight: 47084.17

Authors

Zhu, X.,Wilson, I.A. (deposition date: 2013-12-12, release date: 2014-02-19, Last modification date: 2024-10-30)

Primary citation

Grover, R.K.,Zhu, X.,Nieusma, T.,Jones, T.,Boero, I.,MacLeod, A.S.,Mark, A.,Niessen, S.,Kim, H.J.,Kong, L.,Assad-Garcia, N.,Kwon, K.,Chesi, M.,Smider, V.V.,Salomon, D.R.,Jelinek, D.F.,Kyle, R.A.,Pyles, R.B.,Glass, J.I.,Ward, A.B.,Wilson, I.A.,Lerner, R.A.
A structurally distinct human mycoplasma protein that generically blocks antigen-antibody union.
Science, 343:656-661, 2014

PubMed Abstract: We report the discovery of a broadly reactive antibody-binding protein (Protein M) from human mycoplasma. The crystal structure of the ectodomain of transmembrane Protein M differs from other known protein structures, as does its mechanism of antibody binding. Protein M binds with high affinity to all types of human and nonhuman immunoglobulin G, predominantly through attachment to the conserved portions of the variable region of the κ and λ light chains. Protein M blocks antibody-antigen union, likely because of its large C-terminal domain extending over the antibody-combining site, blocking entry to large antigens. Similar to the other immunoglobulin-binding proteins such as Protein A, Protein M as well as its orthologs in other Mycoplasma species could become invaluable reagents in the antibody field.

PubMed: 24503852
DOI: 10.1126/science.1246135

Experimental method

X-RAY DIFFRACTION (1.2 Å)