4NZU
Crystal structure of the primary monoclonal antibody 13PL Fab' from a multiple myeloma patient
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97945 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.179, 80.403, 102.607 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.287 - 1.200 |
R-factor | 0.13721 |
Rwork | 0.136 |
R-free | 0.16333 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hil |
RMSD bond length | 0.008 |
RMSD bond angle | 1.340 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.287 | 1.220 |
High resolution limit [Å] | 1.200 | 1.200 |
Number of reflections | 126314 | |
<I/σ(I)> | 27.4 | 1.2 |
Completeness [%] | 95.1 | 73.7 |
Redundancy | 6.2 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 295 | 0.1 M citric acid, pH 4.0, 1.0 M lithium chloride, 23% PEG6000, VAPOR DIFFUSION, SITTING DROP, temperature 295K |