4NY6
Neutron structure of leucine and valine methyl protonated type III antifreeze
Summary for 4NY6
| Entry DOI | 10.2210/pdb4ny6/pdb |
| Related | 3QF6 |
| Descriptor | Type-3 ice-structuring protein HPLC 12 (2 entities in total) |
| Functional Keywords | antifreeze protein |
| Biological source | Zoarces americanus (North-Atlantic ocean pout,mother-of-eels,muttonfish,poodler) |
| Total number of polymer chains | 1 |
| Total formula weight | 6894.13 |
| Authors | Fisher, S.J.,Blakeley, M.P.,Howard, E.I.,Petite-Haertlein, I.,Haertlein, M.,Mitschler, A.,Cousido-Siah, A.,Salvaya, A.G.,Popov, A.,Muller-Dieckmann, C.,Petrova, T.,Podjarny, A.D. (deposition date: 2013-12-10, release date: 2014-12-24, Last modification date: 2024-02-28) |
| Primary citation | Fisher, S.J.,Blakeley, M.P.,Howard, E.I.,Petit-Haertlein, I.,Haertlein, M.,Mitschler, A.,Cousido-Siah, A.,Salvay, A.G.,Popov, A.,Muller-Dieckmann, C.,Petrova, T.,Podjarny, A. Perdeuteration: improved visualization of solvent structure in neutron macromolecular crystallography. Acta Crystallogr.,Sect.D, 70:3266-3272, 2014 Cited by PubMed Abstract: The 1.8 Å resolution neutron structure of deuterated type III antifreeze protein in which the methyl groups of leucine and valine residues are selectively protonated is presented. Comparison between this and the 1.85 Å resolution neutron structure of perdeuterated type III antifreeze protein indicates that perdeuteration improves the visibility of solvent molecules located in close vicinity to hydrophobic residues, as cancellation effects between H atoms of the methyl groups and nearby heavy-water molecules (D2O) are avoided. PubMed: 25478844DOI: 10.1107/S1399004714021610 PDB entries with the same primary citation |
| Experimental method | NEUTRON DIFFRACTION (1.85 Å) X-RAY DIFFRACTION (1.05 Å) |
Structure validation
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