4NWP

Computationally Designed Two-Component Self-Assembling Tetrahedral Cage, T33-21, Crystallized in Space Group R32

Summary for 4NWP

• Entry DOI: 10.2210/pdb4nwp/pdb
• Related: 4NWN 4NWO 4NWQ 4NWR
• Descriptor: Putative uncharacterized protein, Uncharacterized protein, SULFATE ION, ... (5 entities in total)
• Functional Keywords: two-component, self-assembling, tetrahedron, designed protein cage, computational design, computational biology, protein engineering, multimerization, nanomaterial, nanostructure, protein binding
• Biological source: Pyrococcus horikoshii; More
• Total number of polymer chains: 8
• Total formula weight: 137359.23

Authors

McNamara, D.E.,King, N.P.,Bale, J.B.,Sheffler, W.,Baker, D.,Yeates, T.O. (deposition date: 2013-12-06, release date: 2014-05-28, Last modification date: 2023-09-20)

Primary citation

King, N.P.,Bale, J.B.,Sheffler, W.,McNamara, D.E.,Gonen, S.,Gonen, T.,Yeates, T.O.,Baker, D.
Accurate design of co-assembling multi-component protein nanomaterials.
Nature, 510:103-108, 2014

PubMed Abstract: The self-assembly of proteins into highly ordered nanoscale architectures is a hallmark of biological systems. The sophisticated functions of these molecular machines have inspired the development of methods to engineer self-assembling protein nanostructures; however, the design of multi-component protein nanomaterials with high accuracy remains an outstanding challenge. Here we report a computational method for designing protein nanomaterials in which multiple copies of two distinct subunits co-assemble into a specific architecture. We use the method to design five 24-subunit cage-like protein nanomaterials in two distinct symmetric architectures and experimentally demonstrate that their structures are in close agreement with the computational design models. The accuracy of the method and the number and variety of two-component materials that it makes accessible suggest a route to the construction of functional protein nanomaterials tailored to specific applications.

PubMed: 24870237
DOI: 10.1038/nature13404

Experimental method

X-RAY DIFFRACTION (2.1 Å)