4NUG

Crystal structure of HIV-1 broadly neutralizing antibody PGT151

4NUG の概要

• エントリーDOI: 10.2210/pdb4nug/pdb
• 分子名称: PGT151 light chain, PGT151 heavy chain, HEXAETHYLENE GLYCOL, ... (4 entities in total)
• 機能のキーワード: immunoglobulin, fab fragment, immune system, hiv envelope
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 50709.91

構造登録者

Blattner, C.,Wilson, I.A. (登録日: 2013-12-03, 公開日: 2014-05-14, 最終更新日: 2024-10-30)

主引用文献

Blattner, C.,Lee, J.H.,Sliepen, K.,Derking, R.,Falkowska, E.,de la Pena, A.T.,Cupo, A.,Julien, J.P.,van Gils, M.,Lee, P.S.,Peng, W.,Paulson, J.C.,Poignard, P.,Burton, D.R.,Moore, J.P.,Sanders, R.W.,Wilson, I.A.,Ward, A.B.
Structural Delineation of a Quaternary, Cleavage-Dependent Epitope at the gp41-gp120 Interface on Intact HIV-1 Env Trimers.
Immunity, 40:669-680, 2014

PubMed Abstract: All previously characterized broadly neutralizing antibodies to the HIV-1 envelope glycoprotein (Env) target one of four major sites of vulnerability. Here, we define and structurally characterize a unique epitope on Env that is recognized by a recently discovered family of human monoclonal antibodies (PGT151-PGT158). The PGT151 epitope is comprised of residues and glycans at the interface of gp41 and gp120 within a single protomer and glycans from both subunits of a second protomer and represents a neutralizing epitope that is dependent on both gp120 and gp41. Because PGT151 binds only to properly formed, cleaved trimers, this distinctive property, and its ability to stabilize Env trimers, has enabled the successful purification of mature, cleaved Env trimers from the cell surface as a complex with PGT151. Here we compare the structural and functional properties of membrane-extracted Env trimers from several clades with those of the soluble, cleaved SOSIP gp140 trimer.

PubMed: 24768348
DOI: 10.1016/j.immuni.2014.04.008

実験手法

X-RAY DIFFRACTION (1.8617 Å)