4NSR

Crystal structure of the Immunity protein

Summary for 4NSR

• Entry DOI: 10.2210/pdb4nsr/pdb
• Related: 4NSO
• Descriptor: Immunity protein (2 entities in total)
• Functional Keywords: helix, peptidoglycan, protein binding
• Biological source: Vibrio cholerae O1 biovar El Tor
• Total number of polymer chains: 6
• Total formula weight: 79601.06

Authors

Dong, C.,Gao, Z.-Q.,Dong, Y.-H. (deposition date: 2013-11-28, release date: 2014-04-16, Last modification date: 2024-02-28)

Primary citation

Zhang, J.,Zhang, H.,Gao, Z.,Hu, H.,Dong, C.,Dong, Y.H.
Structural basis for recognition of the type VI spike protein VgrG3 by a cognate immunity protein.
Febs Lett., 588:1891-1898, 2014

PubMed Abstract: The bacterial type VI secretion system (T6SS) is used by donor cells to inject toxic effectors into receptor cells. The donor cells produce the corresponding immunity proteins to protect themselves against the effector proteins, thereby preventing their self-intoxication. Recently, the C-terminal domain of VgrG3 was identified as a T6SS effector. Information on the molecular mechanism of VgrG3 and its immunity protein TsaB has been lacking. Here, we determined the crystal structures of native TsaB and the VgrG3C-TsaB complex. VgrG3C adopts a canonical phage-T4-lysozyme-like fold. TsaB interacts with VgrG3C through molecular mimicry, and inserts into the VgrG3C pocket.

PubMed: 24751834
DOI: 10.1016/j.febslet.2014.04.016

Experimental method

X-RAY DIFFRACTION (2.795 Å)