4NSO

Crystal structure of the effector-immunity protein complex

4NSO の概要

• エントリーDOI: 10.2210/pdb4nso/pdb
• 関連するPDBエントリー: 4NSR
• 分子名称: Effector protein, Immunity protein (3 entities in total)
• 機能のキーワード: helix, peptidoglycan, protein binding
• 由来する生物種: Vibrio cholerae O1 biovar El Tor; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 46841.88

構造登録者

Dong, C. (登録日: 2013-11-28, 公開日: 2014-04-16, 最終更新日: 2024-10-30)

主引用文献

Zhang, J.,Zhang, H.,Gao, Z.,Hu, H.,Dong, C.,Dong, Y.H.
Structural basis for recognition of the type VI spike protein VgrG3 by a cognate immunity protein.
Febs Lett., 588:1891-1898, 2014

PubMed Abstract: The bacterial type VI secretion system (T6SS) is used by donor cells to inject toxic effectors into receptor cells. The donor cells produce the corresponding immunity proteins to protect themselves against the effector proteins, thereby preventing their self-intoxication. Recently, the C-terminal domain of VgrG3 was identified as a T6SS effector. Information on the molecular mechanism of VgrG3 and its immunity protein TsaB has been lacking. Here, we determined the crystal structures of native TsaB and the VgrG3C-TsaB complex. VgrG3C adopts a canonical phage-T4-lysozyme-like fold. TsaB interacts with VgrG3C through molecular mimicry, and inserts into the VgrG3C pocket.

PubMed: 24751834
DOI: 10.1016/j.febslet.2014.04.016

実験手法

X-RAY DIFFRACTION (2.4 Å)