4NSO

Crystal structure of the effector-immunity protein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-10
Detector	MAR CCD 165 mm
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	133.631, 78.660, 47.860
Unit cell angles	90.00, 101.52, 90.00

Refinement procedure

Resolution	38.165 - 2.400
R-factor	0.2199
Rwork	0.215
R-free	0.25900
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.048
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.073	0.548
Number of reflections	19076
<I/σ(I)>		2.9
Completeness [%]		98.1
Redundancy	7.5	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	277	0.1mM HEPES pH7.6, 12%(w/v)PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 277K