4NJ7

PB1 Domain of AtARF7 - SeMet Derivative

4NJ7 の概要

• エントリーDOI: 10.2210/pdb4nj7/pdb
• 関連するPDBエントリー: 4NJ6
• 分子名称: Auxin response factor 7 (1 entity in total)
• 機能のキーワード: pb1 domain, beta-grasp fold, protein binding
• 由来する生物種: Arabidopsis thaliana (mouse-ear cress,thale-cress)
• 細胞内の位置: Nucleus: P93022
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 177196.72

構造登録者

Korasick, D.A.,Westfall, C.S.,Lee, S.G.,Nanao, M.,Jez, J.M.,Strader, L.C. (登録日: 2013-11-08, 公開日: 2014-03-26, 最終更新日: 2024-11-06)

主引用文献

Korasick, D.A.,Westfall, C.S.,Lee, S.G.,Nanao, M.H.,Dumas, R.,Hagen, G.,Guilfoyle, T.J.,Jez, J.M.,Strader, L.C.
Molecular basis for AUXIN RESPONSE FACTOR protein interaction and the control of auxin response repression.
Proc.Natl.Acad.Sci.USA, 111:5427-5432, 2014

PubMed Abstract: In plants, the AUXIN RESPONSE FACTOR (ARF) transcription factor family regulates gene expression in response to auxin. In the absence of auxin, ARF transcription factors are repressed by interaction with AUXIN/INDOLE 3-ACETIC ACID (Aux/IAA) proteins. Although the C termini of ARF and Aux/IAA proteins facilitate their homo- and heterooligomerization, the molecular basis for this interaction remained undefined. The crystal structure of the C-terminal interaction domain of Arabidopsis ARF7 reveals a Phox and Bem1p (PB1) domain that provides both positive and negative electrostatic interfaces for directional protein interaction. Mutation of interface residues in the ARF7 PB1 domain yields monomeric protein and abolishes interaction with both itself and IAA17. Expression of a stabilized Aux/IAA protein (i.e., IAA16) bearing PB1 mutations in Arabidopsis suggests a multimerization requirement for ARF protein repression, leading to a refined auxin-signaling model.

PubMed: 24706860
DOI: 10.1073/pnas.1400074111

実験手法

X-RAY DIFFRACTION (3 Å)