Crystal structure of human insulin mutant B20 D-ala, B23 D-ala

Summary for 4NIB

DescriptorInsulin A chain, Insulin B chain (3 entities in total)
Functional Keywordshormone
Biological sourceHomo sapiens (human)
Cellular locationSecreted P01308 P01308
Total number of polymer chains2
Total molecular weight5845.7
Menting, J.G.,Lawrence, M.C. (deposition date: 2013-11-05, release date: 2014-08-27, Last modification date: 2015-05-06)
Primary citation
Menting, J.G.,Yang, Y.,Chan, S.J.,Phillips, N.B.,Smith, B.J.,Whittaker, J.,Wickramasinghe, N.P.,Whittaker, L.J.,Pandyarajan, V.,Wan, Z.L.,Yadav, S.P.,Carroll, J.M.,Strokes, N.,Roberts, C.T.,Ismail-Beigi, F.,Milewski, W.,Steiner, D.F.,Chauhan, V.S.,Ward, C.W.,Weiss, M.A.,Lawrence, M.C.
Protective hinge in insulin opens to enable its receptor engagement.
Proc.Natl.Acad.Sci.USA, 111:E3395-E3404, 2014
PubMed: 25092300 (PDB entries with the same primary citation)
DOI: 10.1073/pnas.1412897111
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.1640002.0%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 4nib
no rotation
Molmil generated image of 4nib
rotated about x axis by 90°
Molmil generated image of 4nib
rotated about y axis by 90°