4N78

The WAVE Regulatory Complex Links Diverse Receptors to the Actin Cytoskeleton

4N78 の概要

• エントリーDOI: 10.2210/pdb4n78/pdb
• 関連するPDBエントリー: 3P8C
• 分子名称: Cytoplasmic FMR1-interacting protein 1, Nck-associated protein 1, Wiskott-Aldrich syndrome protein family member 1, ... (9 entities in total)
• 機能のキーワード: actin dynamics, protein binding
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm, perinuclear region (By similarity): Q7L576; Cell membrane; Single-pass membrane protein; Cytoplasmic side (By similarity): Q9Y2A7; Cytoplasm, cytoskeleton: Q92558; Cytoplasm, cytoskeleton (By similarity): J3KNB2
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 403900.56

構造登録者

Chen, Z.C. (登録日: 2013-10-15, 公開日: 2014-02-05, 最終更新日: 2024-02-28)

主引用文献

Chen, B.,Brinkmann, K.,Chen, Z.,Pak, C.W.,Liao, Y.,Shi, S.,Henry, L.,Grishin, N.V.,Bogdan, S.,Rosen, M.K.
The WAVE Regulatory Complex Links Diverse Receptors to the Actin Cytoskeleton.
Cell(Cambridge,Mass.), 156:195-207, 2014

PubMed Abstract: The WAVE regulatory complex (WRC) controls actin cytoskeletal dynamics throughout the cell by stimulating the actin-nucleating activity of the Arp2/3 complex at distinct membrane sites. However, the factors that recruit the WRC to specific locations remain poorly understood. Here, we have identified a large family of potential WRC ligands, consisting of ∼120 diverse membrane proteins, including protocadherins, ROBOs, netrin receptors, neuroligins, GPCRs, and channels. Structural, biochemical, and cellular studies reveal that a sequence motif that defines these ligands binds to a highly conserved interaction surface of the WRC formed by the Sra and Abi subunits. Mutating this binding surface in flies resulted in defects in actin cytoskeletal organization and egg morphology during oogenesis, leading to female sterility. Our findings directly link diverse membrane proteins to the WRC and actin cytoskeleton and have broad physiological and pathological ramifications in metazoans.

PubMed: 24439376
DOI: 10.1016/j.cell.2013.11.048

実験手法

X-RAY DIFFRACTION (2.43 Å)