4N4Z

Trypanosoma brucei procathepsin B structure solved by Serial Microcrystallography using synchrotron radiation

Summary for 4N4Z

• Entry DOI: 10.2210/pdb4n4z/pdb
• Related: 4HWY
• Descriptor: Cysteine peptidase C (CPC), 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• Functional Keywords: papain fold, hydrolase, propeptide, glycosylation
• Biological source: Trypanosoma brucei brucei
• Total number of polymer chains: 1
• Total formula weight: 38270.63

Authors

Gati, C.,Bourenkov, G.,Klinge, M.,Rehders, D.,Stellato, F.,Oberthuer, D.,White, T.A.,Yevanov, O.,Sommer, B.P.,Mogk, S.,Duszenko, M.,Betzel, C.,Schneider, T.R.,Chapman, H.N.,Redecke, L. (deposition date: 2013-10-08, release date: 2014-02-05, Last modification date: 2024-10-09)

Primary citation

Gati, C.,Bourenkov, G.,Klinge, M.,Rehders, D.,Stellato, F.,Oberthur, D.,Yefanov, O.,Sommer, B.P.,Mogk, S.,Duszenko, M.,Betzel, C.,Schneider, T.R.,Chapman, H.N.,Redecke, L.
Serial crystallography on in vivo grown microcrystals using synchrotron radiation.
IUCrJ, 1:87-94, 2014

PubMed Abstract: Crystal structure determinations of biological macromolecules are limited by the availability of sufficiently sized crystals and by the fact that crystal quality deteriorates during data collection owing to radiation damage. Exploiting a micrometre-sized X-ray beam, high-precision diffractometry and shutterless data acquisition with a pixel-array detector, a strategy for collecting data from many micrometre-sized crystals presented to an X-ray beam in a vitrified suspension is demonstrated. By combining diffraction data from 80 Trypanosoma brucei procathepsin B crystals with an average volume of 9 µm(3), a complete data set to 3.0 Å resolution has been assembled. The data allowed the refinement of a structural model that is consistent with that previously obtained using free-electron laser radiation, providing mutual validation. Further improvements of the serial synchrotron crystallography technique and its combination with serial femtosecond crystallography are discussed that may allow the determination of high-resolution structures of micrometre-sized crystals.

PubMed: 25075324
DOI: 10.1107/S2052252513033939

Experimental method

X-RAY DIFFRACTION (3.3 Å)